Journal: Biomacromolecules

Article Title: Toroidal-Spiral Particles for Codelivery of Anti-VEGFR-2 Antibody and Irinotecan: A Potential Implant to Hinder Recurrence of Glioblastoma Multiforme

doi: 10.1021/bm401550r

Figure Lengend Snippet: Flow cytometry measurements of anti-VEGFR-2 antibody binding to HUVECs. (a) Anti-VEGFR-2 antibody released from TS channels without alginate showed similar fluorescence from the cells for time points after 3 h (representative data acquired from one sample run). (b) Percent cells bound with the antibody were similar for release times beyond three hours. (c) For TSPs fabricated with alginate in the channels, the fluorescence from the cells increased with longer time of release up to 72 h (representative data acquired from one sample run). (d) The percent cells bound with the antibody increased over time up to 72 h. The isotype control for each case showed minimal binding. Each data point represents the mean plus or minus the standard deviation of three independent samples.

Article Snippet: Cells were then centrifuged for 5 min at 1000 rpm and the supernatant was removed, followed by two stages of washing with 500 μL of FACS buffer to remove unbound anti-VEGRF-2 antibody., The cell suspension was added into flow cytometry tubes (BD Falcon 12 × 75 mm tube with 35 μL cell strainer cap) and analyzed using a flow cytometer (CyAn ADP, Beckman Coulter, Fullerton, CA) with Summit software v4.3 (Beckman Coulter, Fullerton, CA).

Techniques: Flow Cytometry, Binding Assay, Fluorescence, Control, Standard Deviation